Personal protective equipment is non-negotiable. Cryogenic gloves rated to minus 196 degrees Celsius, full face shield, closed-toe shoes, and a lab coat are the minimum standard. In enclosed rooms, a fixed or portable oxygen depletion monitor is essential because liquid nitrogen evaporation rapidly displaces breathable oxygen without any visible or olfactory warning. Cryolab recommends that all staff handling liquid nitrogen dewars complete formal cryogenic safety training and that emergency procedures are posted at every storage location.