Sperm motility analysis before cryopreservation is essential for multiple clinical reasons. It determines whether the sample meets the quality threshold to be worth freezing. It informs the dilution ratio with cryoprotectant solution. It establishes a baseline for comparing post-thaw survival. And it forms part of the patient’s permanent fertility record, which is significant when the sample is used years later. Using calibrated sperm analysis equipment or a CASA system for this assessment ensures the data is accurate and reproducible rather than subject to observer variation.