Anyone working in or around a UK IVF laboratory who handles liquid nitrogen requires appropriate cryogenic safety training. This is a legal requirement under the Control of Substances Hazardous to Health regulations, which classify liquid nitrogen as a hazardous substance. The training requirement extends beyond embryologists to include any support or facilities staff who handle liquid nitrogen deliveries.
Core topics that cryogenic safety training should cover include:
The physical properties and hazards of liquid nitrogen, including the asphyxiation risk from nitrogen gas accumulation, the cryogenic burn risk from liquid contact, and the pressure hazards if liquid nitrogen is trapped in a sealed container.
The correct use of personal protective equipment, including cryogenic gloves, face shields, and appropriate footwear. The difference between standard laboratory PPE and cryogenic-rated PPE must be clearly communicated.
The operation of oxygen depletion monitoring systems and the correct response to an oxygen alarm, including evacuation procedures and the requirement never to enter a room with a triggered oxygen alarm without breathing apparatus.
Emergency first aid for cryogenic burns and for suspected nitrogen asphyxiation.
Dewar handling and top-up procedures, including the correct method for transferring liquid nitrogen between vessels.
Training records must be maintained for all relevant staff. Refresher training should be scheduled periodically, typically annually for staff with regular nitrogen handling responsibilities.
Cryolab can provide guidance on cryogenic safety documentation and training frameworks. Visit cryolab.co.uk“>Cryolab
