Vit kit generally contains a high security straw, a capillary tube with a pre-formed gutter, coloured handling rods, and insertion devices. To use the kit, prepare your samples according to laboratory protocols, then carefully load each sample into the straw using the handling rod. The pre-formed gutter ensures precise placement and prevents contamination or sample loss. Once loaded, the samples are rapidly cooled through vitrification, which avoids ice crystal formation and preserves cellular integrity. After vitrification, the straw can be securely sealed using compatible manual or automatic sealers and stored safely in liquid nitrogen tanks or dry shippers for long-term preservation. Proper technique and handling are essential to maintain the viability and quality of the biological samples.