Not exactly, but very close. Here’s what to expect:
Temperature stability:
- ±1°C variation (normal)
- -196°C at fill
- Gradually drifts to -190°C over 6-8 weeks as liquid nitrogen evaporates
- After refill, returns to -196°C
Why the drift? When liquid nitrogen evaporates:
- Gaseous nitrogen (top of dewar) is -160°C to -180°C
- This colder gas (above liquid) insulates the liquid slightly less
- The liquid slowly warms as the level drops
Important note: Even at -190°C, samples remain perfectly viable. The critical threshold is about -150°C, so you have a large safety margin.